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Low temperature photoacoustic spectroscopy of the purple membrane of Halobacterium halobium

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Abstract

The photoacoustic spectra of light- and dark-adapted bacteriorhodopsin in aqueous and mixed water–glycerol (1:2) suspensions are studied in the 90–300 K temperature range. Photochemical transients are observed and interpreted in terms of the known photocycles of trans- and 13-cis-bacteriorhodopsin. The spectra differ from those obtained by low temperature optical absorption spectroscopy of glassy glycerol–water suspensions.

© 1982 Optical Society of America

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