Abstract

Computer-software-controlled placement of cells onto microscope slides is advantageous for quantitative optical microscopy. Cell detection, identification, and placement have been implemented on a software-controlled flow sorter. Computer hardware timing latencies that comprise the dead times of the system are sufficiently short (10 μs) to allow adequate delay of cells for sorting. Operating system timing latencies (150 μs) are also short enough for low flow-rate sorting. As a result of the low cost of computers and because formats for cell placement are still controversial, software control is desirable since it provides the flexibility to implement multiple variations for cell detection, identification, and sorting.

© 1987 Optical Society of America

Automated single-cell manipulation and sorting by light trapping

Tudor N. Buican, Miriam J. Smyth, Harry A. Crissman, Gary C. Salzman, Carleton C. Stewart, and John C. Martin
Appl. Opt. 26(24) 5311-5316 (1987)

Multiprocessing interval processor for automated cytogenetics

David Lloyd, Jim Piper, Denis Rutovitz, and Geoffrey Shippey
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Automated densitometry of cell populations in a continuous-motion imaging cell scanner

James H. Tucker, O. A. N. Husain, Keith Watts, Stephen Farrow, Royston Bayley, and Margaret H. Stark
Appl. Opt. 26(16) 3315-3324 (1987)

Optical automation for sputum cytology

Paul R. Swank and S. Donald Greenberg
Appl. Opt. 26(16) 3373-3378 (1987)

High performance pathology workstation using an automated multispectral microscope

Harvey L. Kasdan, Kenneth C. Langford, John Liberty, Michael Zachariash, and Fred H. Deindoerfer
Appl. Opt. 26(16) 3294-3300 (1987)