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A Spectrofluorophosphorimeter

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Abstract

An instrument is described which allows the determination of fluorescence and phosphorescence spectra, fluorescence and phosphorescence excitation spectra, and the decay of triplet states involved in phosphorescence. A variable chopping speed (0–6000 rpm) allows determination of lifetimes down to 0.1 msec. Fluctuations in the 450-W light source are compensated by a quantum counter and a ratio divider, which plots the ratio of signals from the light emitter and the quantum counter. The instrument has been calibrated so that wavelength variations in excitation intensity, bandwidth, monochromator transmission, and phototube sensitivity are corrected, and only the number of quanta emitted by a sample at each wavelength is reported. Examples are given of the spectra of Rhodamine B and eosin.

© 1964 Optical Society of America

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