Abstract

The sensitivity limit for one-lane DNA sequencing based on laser-induced fluorescence is derived by statistical means. A closed-form expression for the sequencing error probability as a function of the signal strength and of the differences in the fluorescence properties of the labels is given. Fluctuations of the signal caused by photodestruction of the molecules cannot be neglected. The expected sequencing error probability is expressed in terms of the number of molecules and the physical parameters of the measurement. This equation can be used in many ways to design and optimize sequencing experiments.

© 1993 Optical Society of America

Semiconductor laser-induced fluorescence detection in picoliter volume flow cells

Anders P. Larson, Henrik Ahlberg, and Staffan Folestad
Appl. Opt. 32(6) 794-805 (1993)

Effects of fluorescence excitation geometry on the accuracy of DNA fragment sizing by flow cytometry

James H. Werner, Erica J. Larson, Peter M. Goodwin, W. Patrick Ambrose, and Richard A. Keller
Appl. Opt. 39(16) 2831-2839 (2000)

Detection of trace amounts of Cr by two laser-based spectroscopic techniques: laser-enhanced ionization in flames and laser-induced fluorescence in graphite furnace

Ove Axner and Halina Rubinsztein-Dunlop
Appl. Opt. 32(6) 867-884 (1993)

Photolysis–laser-induced fluorescence diagnostic for GaCl

Angelo J. Alfano and David J. Benard
Appl. Opt. 32(27) 5373-5378 (1993)

Maximum-likelihood criterion and single-molecule detection

Jörg Enderlein
Appl. Opt. 34(3) 514-526 (1995)