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Quantitative phase imaging in confocal microscopy by optical differentiation

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Abstract

The technique of optical differentiation is applied to confocal microscopy for the purpose of quantitative phase imaging. One-dimensional absorptive filters are placed in the pupil of the microscope objective to produce images related to the local phase slope in the object. With suitable signal processing and integration, a quantitative phase profile is obtained. This method is demonstrated in a reflection-based surface-profiling instrument.

© 1994 Optical Society of America

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