Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Real-time scanning slit confocal microscopy of the in vivohuman cornea

Not Accessible

Your library or personal account may give you access

Abstract

We describe a new, nonapplanating, real-time scanning slit confocal microscope that produces unique real-time video images of the in vivo human cornea. This new real-time slit scanning confocal microscope produces en face, single-video-frame images (2-μm sections) with high contrast through the full thickness (500 μm) of the normal cornea. No frame averaging or digital image enhancement is required. The images of superficial epithelial cells, wing cells, basal epithelial cells, corneal innervation, nuclei of stromal keratocytes, and the cell bodies of the stromal keratocytes in the posterior stromal region demonstrate the unique real-time imaging characteristics. The microscope is equipped with water-immersion microscope objectives of high numerical aperture. The microscope objective does not flatten or distort the cornea; a polymer gel is used to couple the microscope objective optically to the cornea.

© 1994 Optical Society of America

Full Article  |  PDF Article
More Like This
Real-time confocal laser scan microscope for examination and diagnosis of the eye in vivo

J. H. Massig, M. Preissler, A. R. Wegener, and G. Gaida
Appl. Opt. 33(4) 690-694 (1994)

Three-dimensional reconstruction of the rabbit cornea by confocal scanning optical microscopy and volume rendering

Barry R. Masters and Stephen W. Paddock
Appl. Opt. 29(26) 3816-3822 (1990)

Clinical microscopy of the cornea utilizing optical sectioning and a high-numerical-aperture objective

Charles J. Koester, James D. Auran, Heinz D. Rosskothen, George J. Florakis, and Robert B. Tackaberry
J. Opt. Soc. Am. A 10(7) 1670-1679 (1993)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (11)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved