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Two-photon-induced fluorescence from the phycoerythrin protein

Zhongping Chen, D. L. Kaplan, K. Yang, J. Kumar, K. A. Marx, and S. K. Tripathy

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Abstract

Two-photon-induced fluorescence is observed from the photodynamic phycobiliprotein phycoerythrin. Temporal, spectral, and intensity-dependent properties of the two-photon-induced fluorescence emission from phycoerythrin excited by a 1.06-µm laser beam are reported. The measured two-photon absorption cross section of phycoerythrin is an order of magnitude larger than that of Rhodamine 6G. The potential applications of phycobiliproteins for two-photon-induced fluorescence for microscopy of three-dimensional biological samples and three-dimensional optical memory are discussed.

© 1997 Optical Society of America

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