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Confocal microscope with large field and working distance

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Abstract

A confocal microscope that uses separate, noncollinear objective lenses for illumination and collection makes it possible to work 20 mm from a sample with a field of view of 2.25 mm across and still achieve lateral and axial resolutions below 5 µm. The design is expected to have application to in vivo confocal microscopy, allowing a field of view and section thickness similar to those used by pathologists in examining excised tissue.

© 1999 Optical Society of America

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