Abstract

We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.

© 1999 Optical Society of America

Digital holography for quantitative phase-contrast imaging

Etienne Cuche, Frédéric Bevilacqua, and Christian Depeursinge
Opt. Lett. 24(5) 291-293 (1999)

Fast numerical reconstruction technique for high-resolution hybrid holographic microscopy

Yasuhiro Takaki and Hitoshi Ohzu
Appl. Opt. 38(11) 2204-2211 (1999)

Fast-iterative automatic reconstruction method for quantitative phase image with reduced phase perturbations in off-axis digital holographic microscopy

Raul Castaneda and Ana Doblas
Appl. Opt. 60(32) 10214-10220 (2021)

Polarization microscopy by use of digital holography: application to optical-fiber birefringence measurements

Tristan Colomb, Florian Dürr, Etienne Cuche, Pierre Marquet, Hans G. Limberger, René-Paul Salathé, and Christian Depeursinge
Appl. Opt. 44(21) 4461-4469 (2005)

Off-axis digital hologram reconstruction: some practical considerations

Nicolas Verrier and Michael Atlan
Appl. Opt. 50(34) H136-H146 (2011)