Abstract

A pulsed ruby laser coupled to a diffraction-limited microscope has been developed for application to bio-optical research. Theoretical limitations on maximum energy density and minimum spot size are indicated and practical compromises are discussed. Small samples of organic and inorganic materials have been irradiated and vaporized. Single living cells have been exposed to radiation spots of 1 μ to 2 μ in diameter and destroyed at energy densities of about 10²J/mm².

© 1965 Optical Society of America

A Study of Errors in the Measurement of Microscopic Spheres

Charles Proffer Saylor
Appl. Opt. 4(4) 477-486 (1965)

Scanned Laser Infrared Microscope

B. Sherman and J. F. Black
Appl. Opt. 9(4) 802-809 (1970)

Laser Retinal Photocoagulator

N. S. Kapany, N. Silbertrust, and N. A. Peppers
Appl. Opt. 4(5) 517-522 (1965)

Beam Divergence Measurement for Q-Switched Ruby Lasers

R. W. Waynant, J. H. Cullom, I.T. Basil, and G. D. Baldwin
Appl. Opt. 4(12) 1648-1651 (1965)

Scanning Laser Microscope for Biological Investigations

P. Davidovits and M. D. Egger
Appl. Opt. 10(7) 1615-1619 (1971)