Abstract

A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum’s peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 μm at a wavelength of 0.633 μm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 μm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others.

© 2004 Optical Society of America

Calibration-free method to determine the size and hemoglobin concentration of individual red blood cells from light scattering

Konstantin A. Sem’yanov, Pit A. Tarasov, Juhani T. Soini, Alexander K. Petrov, and Valeri P. Maltsev
Appl. Opt. 39(31) 5884-5889 (2000)

Absolute real-time measurement of particle size distribution with the flying light-scattering indicatrix method

V. P. Maltsev, A. V. Chernyshev, K. A. Sem'yanov, and E. Soini
Appl. Opt. 35(18) 3275-3280 (1996)

Light-scattering properties of individual erythrocytes

Alexandr N. Shvalov, Juhani T. Soini, Andrey V. Chernyshev, Peter A. Tarasov, Erkki Soini, and Valeri P. Maltsev
Appl. Opt. 38(1) 230-235 (1999)

Measurement of scattering properties of individual particles with a scanning flow cytometer

A. V. Chernyshev, V. I. Prots, A. A. Doroshkin, and V. P. Maltsev
Appl. Opt. 34(27) 6301-6305 (1995)

Parametric solution of the inverse light-scattering problem for individual spherical particles

Valeri P. Maltsev and Valeri N. Lopatin
Appl. Opt. 36(24) 6102-6108 (1997)