Abstract

Fluorescence correlation spectroscopy (FCS) is a sensitive technique used to probe size, concentration, flow velocity, and reaction kinetics in a dilute solution. Conventional FCS spectrometers achieve this sensitivity at the cost of using bulky optics. We demonstrate a technique that utilizes a single-mode optical fiber of $3.3\mu \text{m}$ mode field diameter to perform FCS measurements. We demonstrate that the technique has adequate sensitivity to perform FCS measurements on fluorescent beads of $13\text{\hspace{0.17em} nm}$ radius, and that the results agree with theoretical predictions. Our method potentially allows FCS to be extended to remote and in vivo applications.

© 2006 Optical Society of America

Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber

Yu-Chung Chang, Jing Yong Ye, Thommey Thomas, Yi Chen, James R. Baker, and Theodore B. Norris
Opt. Express 16(17) 12640-12649 (2008)

Optical-fiber-microsphere for remote fluorescence correlation spectroscopy

Heykel Aouani, Frédérique Deiss, Jérôme Wenger, Patrick Ferrand, Neso Sojic, and Hervé Rigneault
Opt. Express 17(21) 19085-19092 (2009)

Spatial fluorescence cross-correlation spectroscopy

Rodolphe Jaffiol, Yoann Blancquaert, Antoine Delon, and Jacques Derouard
Appl. Opt. 45(6) 1225-1235 (2006)

Confined diffusion in tubular structures analyzed by fluorescence correlation spectroscopy on a mirror

Emilien Etienne, Pierre-François Lenne, James N. Sturgis, and Hervé Rigneault
Appl. Opt. 45(18) 4497-4507 (2006)

Site-specific multipoint fluorescence measurement system with end-capped optical fibers

Woosub Song, Sucbei Moon, Byoung-Cheol Lee, Chul-Seung Park, Dug Young Kim, and Hyuk Sang Kwon
Appl. Opt. 50(20) 3529-3537 (2011)