Abstract

Fluorescence correlation spectroscopy (FCS) is a sensitive technique used to probe size, concentration, flow velocity, and reaction kinetics in a dilute solution. Conventional FCS spectrometers achieve this sensitivity at the cost of using bulky optics. We demonstrate a technique that utilizes a single-mode optical fiber of $3.3\mu \text{m}$ mode field diameter to perform FCS measurements. We demonstrate that the technique has adequate sensitivity to perform FCS measurements on fluorescent beads of $13\text{\hspace{0.17em} nm}$ radius, and that the results agree with theoretical predictions. Our method potentially allows FCS to be extended to remote and in vivo applications.

© 2006 Optical Society of America

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