Abstract
A study of the practicality a simple technique for obtaining time-domain information that uses continuous wave detection of fluorescence is presented. We show that this technique has potential for use in assays for which a change in the lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer, for example, and could be particularly important when one is carrying out such measurements in the scaled- down environment of a lab on a chip (biochip). A rate-equation model is presented that allows an objective analysis to be made of the relative importance of the key measurement parameters: optical saturation of the fluorophore and period of the excitation pulse. An experimental demonstration of the technique that uses a cuvette-based analysis of a carbocyanine dye and for which the excitation source is a wavelength, self-pulsing AlGaInP laser diode is compared with the model.
© 2006 Optical Society of America
Full Article | PDF ArticleMore Like This
Mark D. Holton, Oscar R. Silvestre, Rachel J. Errington, Paul J. Smith, Daniel R. Matthews, Paul Rees, and Huw D. Summers
Opt. Express 17(7) 5205-5216 (2009)
Dongeun Kim, Sucbei Moon, Wonsang Hwang, and Dug Young Kim
Opt. Express 30(9) 14677-14685 (2022)
Ralf H. Mayer, Jeffery S. Reynolds, and Eva M. Sevick-Muraca
Appl. Opt. 38(22) 4930-4938 (1999)