Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells

Not Accessible

Your library or personal account may give you access

Abstract

Two-photon fluorescence microscopy is widely applied to biology and medicine to study both the structure and dynamic processes in living cells. The main issue is the slow acquisition rate due to the point scanning approach limiting the multimodal detection (x,y,z,t). To extend the performances of this powerful technique, we present a time-resolved multifocal multiphoton microscope (MMM) based on laser amplitude splitting. An array of 8×8 foci is created on the sample that gives a direct insight of the fluorescence localization. Four-dimensional (4D) imaging is obtained by combining simultaneous foci scanning, time-gated detection, and z displacement. We illustrate time-resolved MMM capabilities for 4D imaging of a photosensitizer inside living colon cancer cells. The aim of this study is to have a better understanding of the photophysical processes implied in the photosensitizer reactivity.

© 2007 Optical Society of America

Full Article  |  PDF Article
More Like This
Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging

S. Kumar, C. Dunsby, P. A. A. De Beule, D. M. Owen, U. Anand, P. M. P. Lanigan, R. K. P. Benninger, D. M. Davis, M. A. A. Neil, P. Anand, C. Benham, A. Naylor, and P. M. W. French
Opt. Express 15(20) 12548-12561 (2007)

Fluorescence-lifetime imaging with a multifocal two-photon microscope

S. Lévêque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges
Opt. Lett. 29(24) 2884-2886 (2004)

Multifocal multiphoton microscopy (MMM) at a frame rate beyond 600 Hz

Karsten Bahlmann, Peter T. C. So, Michael Kirber, Robert Reich, Bernard Kosicki, William McGonagle, and Karl Bellve
Opt. Express 15(17) 10991-10998 (2007)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved