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Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin: comparison of imaging characteristics between mode-locked Cr:forsterite and Ti:sapphire lasers

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Abstract

Second-harmonic-generation (SHG) microscopy is an interesting new tool for observing dermal collagen fiber in skin. However, conventional SHG microscopy using a mode-locked Ti:sapphire laser suffers from low penetration depth and a slow image acquisition rate caused by scattering and absorption in tissue, making it difficult to use for in vivo applications on human skin. We develop an SHG microscope equipped with a mode-locked Cr:forsterite laser with a long wavelength and compare its imaging characteristics with that of a Ti:sapphire-laser-based SHG microscope for the measurement of dermal collagen fiber in animal and human skins. The results indicate the suitability of the Cr:forsterite laser-based SHG microscope for in vivo imaging of human skin.

© 2009 Optical Society of America

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Supplementary Material (4)

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Media 2: MOV (2540 KB)     
Media 3: MOV (2991 KB)     
Media 4: MOV (3245 KB)     

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