Abstract

A technique is proposed that can be used to calibrate the amplitude of fluorescence spectra so that spectra reported by different laboratories can be compared quantitatively. The technique is based upon the scattering of light by a solution of polystyrene. In order to compare quantitatively data obtained from different spectrofluorimeters, these terms are defined: the spectrofluorimetric sensitivity, the noise-equivalent concentration, and the fluorophotometric reproducibility. The author shows how these quantities can be measured with the polystyrene standard.

© 1966 Optical Society of America

Calibration of Spectrofluorimeters for Measuring Corrected Emission Spectra

W. H. Melhuish
J. Opt. Soc. Am. 52(11) 1256-1258 (1962)

Influence of Scattering on Fluorescence Spectra of Dilute Solutions Obtained with the Aminco–Bowman Spectrophotofluorometer

J. M. Price, M. Kaihara, and H. K. Howerton
Appl. Opt. 1(4) 521-533 (1962)

The Calibration of Scanning Monochromators for the Measurement of Absolute Irradiance

John J. Merrill and R. Gary Layton
Appl. Opt. 5(11) 1818-1822 (1966)

Design and testing of a two-monochromator reference spectrofluorimeter for high-accuracy total radiance factor measurements

Joanne C. Zwinkels, D. S. Gignac, M. Nevins, I. Powell, and A. Bewsher
Appl. Opt. 36(4) 892-902 (1997)

Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs

Alan Earp, Christine E. Hanson, Peter J. Ralph, Vittorio E. Brando, Simon Allen, Mark Baird, Lesley Clementson, Paul Daniel, Arnold G. Dekker, Peter R.C.S. Fearns, John Parslow, Peter G. Strutton, Peter A. Thompson, Mark Underwood, Scarla Weeks, and Martina A. Doblin
Opt. Express 19(27) 26768-26782 (2011)