Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Thin-film optoacoustic transducers for subcellular Brillouin oscillation imaging of individual biological cells

Not Accessible

Your library or personal account may give you access

Abstract

At low frequencies ultrasound is a valuable tool to mechanically characterize and image biological tissues. There is much interest in using high-frequency ultrasound to investigate single cells. Mechanical characterization of vegetal and biological cells by measurement of Brillouin oscillations has been demonstrated using ultrasound in the GHz range. This paper presents a method to extend this technique from the previously reported single-point measurements and line scans into a high-resolution acoustic imaging tool. Our technique uses a three-layered metal-dielectric-metal film as a transducer to launch acoustic waves into the cell we want to study. The design of this transducer and measuring system is optimized to overcome the vulnerability of a cell to the exposure of laser light and heat without sacrificing the signal-to-noise ratio. The transducer substrate shields the cell from the laser radiation, efficiently generates acoustic waves, facilitates optical detection in transmission, and aids with heat dissipation away from the cell. This paper discusses the design of the transducers and instrumentation and presents Brillouin frequency images on phantom, fixed, and living cells.

© 2015 Optical Society of America

Full Article  |  PDF Article
More Like This
Time resolved Brillouin fiber-spectrometer

Salvatore La Cavera, Fernando Pérez-Cota, Rafael Fuentes-Domínguez, Richard J. Smith, and Matt Clark
Opt. Express 27(18) 25064-25071 (2019)

High acoustic strains in Si through ultrafast laser excitation of Ti thin-film transducers

Eirini Tzianaki, Makis Bakarezos, George D. Tsibidis, Yannis Orphanos, Panagiotis A. Loukakos, Constantine Kosmidis, Panos Patsalas, Michael Tatarakis, and Nektarios A. Papadogiannis
Opt. Express 23(13) 17191-17204 (2015)

Heterodyne Brillouin microscopy for biomechanical imaging

Michael A. Taylor, Amanda W. Kijas, Zhao Wang, Jan Lauko, and Alan E. Rowan
Biomed. Opt. Express 12(10) 6259-6268 (2021)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (12)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved