Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Feasibility study of high spatial resolution multimodality fluorescence tomography in ex vivo biological tissue

Not Accessible

Your library or personal account may give you access

Abstract

Previously, we demonstrated that temperature-modulated fluorescence tomography (TM-FT) could provide fluorescence images with high quantitative accuracy and the spatial resolution of focused ultrasound. TM-FT is based on scanning the focused ultrasound across the medium to activate temperature-reversible fluorescent nanoprobes (ThermoDots). This technique can resolve small fluorescent targets located several centimeters deep in turbid media with millimeter resolution. Our past studies with this multimodality technique used agar phantoms, which could not represent the true heterogeneous nature of the acoustic and optical properties of biological tissue. In this work, we report the results of the first TM-FT study performed on ex vivo chicken breast tissue. In order to improve the spatial resolution of this technique, diffuse optical tomography is also used to better estimate the optical property maps of the tissue, which is utilized as functional a priori for the TM-FT reconstruction algorithm. These ex vivo results show that TM-FT can accurately recover the concentration and position of a 1.5mm×5mm inclusion filled with ThermoDots. Since the inclusion is embedded 2 cm deep in the chicken breast sample, these results demonstrate the great potential of TM-FT for future in vivo small animal imaging.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Experimental evaluation of the resolution and quantitative accuracy of temperature-modulated fluorescence tomography

Tiffany C. Kwong, Farouk Nouizi, Yuting Lin, Jaedu Cho, Yue Zhu, Uma Sampathkumaran, and Gultekin Gulsen
Appl. Opt. 56(3) 521-529 (2017)

Simulation-based evaluation of the resolution and quantitative accuracy of temperature-modulated fluorescence tomography

Yuting Lin, Farouk Nouizi, Tiffany C. Kwong, and Gultekin Gulsen
Appl. Opt. 54(25) 7612-7621 (2015)

Quantitative fluorescence tomography with functional and structural a priori information

Yuting Lin, Han Yan, Orhan Nalcioglu, and Gultekin Gulsen
Appl. Opt. 48(7) 1328-1336 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved