Application of spatially modulated near-infrared structured light to study changes in optical properties of mouse brain tissue during heatstress

Oren Shaul; Michal Fanrazi-Kahana; Omri Meitav; Gad A. Pinhasi; David Abookasis

doi:10.1364/AO.56.008880

Applied Optics
Vol. 56,
Issue 32,
pp. 8880-8886
(2017)
•https://doi.org/10.1364/AO.56.008880

Application of spatially modulated near-infrared structured light to study changes in optical properties of mouse brain tissue during heatstress

Oren Shaul, Michal Fanrazi-Kahana, Omri Meitav, Gad A. Pinhasi, and David Abookasis

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Oren Shaul, Michal Fanrazi-Kahana, Omri Meitav, Gad A. Pinhasi, and David Abookasis, "Application of spatially modulated near-infrared structured light to study changes in optical properties of mouse brain tissue during heatstress," Appl. Opt. 56, 8880-8886 (2017)

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- Medical Optics and Biotechnology
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About this Article
History
- Original Manuscript: July 18, 2017
- Revised Manuscript: September 3, 2017
- Manuscript Accepted: October 5, 2017
- Published: November 3, 2017
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Virtual Journal for Biomedical Optics Vol. 13, Iss. 1

Abstract

Heat stress (HS) is a medical emergency defined by abnormally elevated body temperature that causes biochemical, physiological, and hematological changes. The goal of the present research was to detect variations in optical properties (absorption, reduced scattering, and refractive index coefficients) of mouse brain tissue during HS by using near-infrared (NIR) spatial light modulation. NIR spatial patterns with different spatial phases were used to differentiate the effects of tissue scattering from those of absorption. Decoupling optical scattering from absorption enabled the quantification of a tissue’s chemical constituents (related to light absorption) and structural properties (related to light scattering). Technically, structured light patterns at low and high spatial frequencies of six wavelengths ranging between 690 and 970 nm were projected onto the mouse scalp surface while diffuse reflected light was recorded by a CCD camera positioned perpendicular to the mouse scalp. Concurrently to pattern projection, brain temperature was measured with a thermal camera positioned slightly off angle from the mouse head while core body temperature was monitored by thermocouple probe. Data analysis demonstrated variations from baseline measurements in a battery of intrinsic brain properties following HS.

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	690 nm		780 nm		800 nm		880 nm		920 nm		970 nm
	28°C	43°C	28°C	43°C	28°C	43°C	28°C	43°C	28°C	43°C	28°C	43°C
Measured	$2.01 \pm 0.03$	$1.88 \pm 0.01$	$1.94 \pm 0.02$	$1.87 \pm 0.04$	$1.63 \pm 0.01$	$1.59 \pm 0.03$	$1.50 \pm 0.04$	$1.48 \pm 0.05$	$1.52 \pm 0.03$	$1.51 \pm 0.04$	$1.59 \pm 0.02$	$1.59 \pm 0.02$
Cauchy	1.71	1.65	1.71	1.65	1.71	1.65	1.71	1.65	1.71	1.65	1.71	1.65
Sellemier	2.12	1.88	1.85	1.72	1.80	1.69	1.61	1.59	1.53	1.54	1.43	1.49
Cornu	2.10	1.90	1.78	1.71	1.74	1.68	1.60	1.58	1.55	1.55	1.50	1.51
Conrady	2.05	1.88	1.82	1.72	1.77	1.69	1.61	1.59	1.54	1.54	1.47	1.49

	28°C	43°C
Cauchy	$A = 1.71$ $B = 9.63$ $C = - 0.4$	$A = 1.65$ $B = 9.64$ $C = - 0.4$
Sellemier	$B 1 = 1999.8$ $C 1 = 310.2$ $B 2 = - 1201.3$ $C 2 = - 1654.4$	$B 1 = - 789.84$ $C 1 = - 785.50$ $B 2 = 789.77$ $C 2 = 794.40$
Cornu	$A = 1.07$ $B = 208.04$ $C = 488.41$	$A = 1.13$ $B = 211.82$ $C = 414.99$
Conrady	$A = 0.01$ $B = 1407.3$ $C = - 0.4$	$A = 0.55$ $B = 917.89$ $C = 9.44$

	690 nm		780 nm		800 nm		880 nm		920 nm		970 nm
	28°C	43°C	28°C	43°C	28°C	43°C	28°C	43°C	28°C	43°C	28°C	43°C
Measured	$2.01 \pm 0.03$	$1.88 \pm 0.01$	$1.94 \pm 0.02$	$1.87 \pm 0.04$	$1.63 \pm 0.01$	$1.59 \pm 0.03$	$1.50 \pm 0.04$	$1.48 \pm 0.05$	$1.52 \pm 0.03$	$1.51 \pm 0.04$	$1.59 \pm 0.02$	$1.59 \pm 0.02$
Cauchy	1.71	1.65	1.71	1.65	1.71	1.65	1.71	1.65	1.71	1.65	1.71	1.65
Sellemier	2.12	1.88	1.85	1.72	1.80	1.69	1.61	1.59	1.53	1.54	1.43	1.49
Cornu	2.10	1.90	1.78	1.71	1.74	1.68	1.60	1.58	1.55	1.55	1.50	1.51
Conrady	2.05	1.88	1.82	1.72	1.77	1.69	1.61	1.59	1.54	1.54	1.47	1.49

	28°C	43°C
Cauchy	$A = 1.71$ $B = 9.63$ $C = - 0.4$	$A = 1.65$ $B = 9.64$ $C = - 0.4$
Sellemier	$B 1 = 1999.8$ $C 1 = 310.2$ $B 2 = - 1201.3$ $C 2 = - 1654.4$	$B 1 = - 789.84$ $C 1 = - 785.50$ $B 2 = 789.77$ $C 2 = 794.40$
Cornu	$A = 1.07$ $B = 208.04$ $C = 488.41$	$A = 1.13$ $B = 211.82$ $C = 414.99$
Conrady	$A = 0.01$ $B = 1407.3$ $C = - 0.4$	$A = 0.55$ $B = 917.89$ $C = 9.44$

Abstract

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Applied Optics