Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Mathematical modeling and experimental validation for expression microdissection

Abstract

Using laser excitation, expression microdissection (xMD) can selectively heat cancer cells targeted via immunohistochemical staining to enable their selective retrieval from tumor tissue samples, thus reducing misdiagnoses caused by contamination of noncancerous cells. Several theoretical models have been validated for the photothermal effect in highly light absorbing and scattering media. However, these models are not generally applicable to the physics behind the process of xMD. In this study, we propose a thermal model that can analyze the transient temperature distribution and heat melt zone in an xMD sample medium composed of a thermoplastic film and a tumor tissue sample sandwiched between two glass slides. Furthermore, we experimentally examined the model using an ink layer with controllable optical properties to serve as a microscale-thin, tissue-mimicking phantom and found the experimentally measured film temperature is in good agreement with the model predictions. The validated model can help researchers to optimize cell retrieval by xMD for improved diagnostics of cancer and other diseases.

© 2020 Optical Society of America

Full Article  |  PDF Article
More Like This
Thermal modeling of Laser Capture Microdissection

Seth R. Goldstein, Philip G. McQueen, and Robert F. Bonner
Appl. Opt. 37(31) 7378-7391 (1998)

Experimental evaluation of mathematical models for predicting the thermal response of tissue to laser irradiation

Jorge H. Torres, Massoud Motamedi, John A. Pearce, and A. J. Welch
Appl. Opt. 32(4) 597-606 (1993)

Experimental validation and mathematical simulation for laser protection performance of light field imaging

Yangliang Li, Qing Ye, Yunlong Wu, Hao Zhang, Haoqi Luo, Ke Sun, and Xiaoquan Sun
Appl. Opt. 62(36) 9621-9630 (2023)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved