Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Single-shot common-path off-axis dual-wavelength digital holographic microscopy

Abstract

A single-shot common-path off-axis self-interference dual-wavelength digital holographic microscopic (DHM) system based on a cube beam splitter is demonstrated to expand the phase range in a stepped microstructure and for simultaneous measurement of the refractive index and physical thickness of a specimen. In the system, two laser beams with wavelengths of 532 nm and 632.8 nm are used. These laser beams are combined to transilluminate the object under study, then the object beam is divided into two beams by using a beam splitter oriented in such a way that both the beams propagate in almost the same direction, with an appropriate lateral separation between them. One of the object beams is spatially filtered at its Fourier plane, using a pinhole to generate a reference spherical beam free from the object information. The reference beam interferes with the object beam to form a digital hologram at the faceplate of the image sensor. The phase information is extracted from a single recorded digital hologram using the phase aberration compensation method that is based on principal component analysis (PCA). Owing to the common-path configuration, the system shows high temporal phase stability and it is less vibration-sensitive compared to counterparts such as a Mach–Zehnder type DHM. The performance of the dual-wavelength DHM system is verified in two different application fields by conducting the experiments using microsphere beads and living plant cells.

© 2020 Optical Society of America

Full Article  |  PDF Article
More Like This
Single-shot common-path off-axis digital holography: applications in bioimaging and optical metrology [Invited]

Manoj Kumar, Osamu Matoba, Xiangyu Quan, Sudheesh K. Rajput, Yasuhiro Awatsuji, and Yosuke Tamada
Appl. Opt. 60(4) A195-A204 (2021)

Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy

Meng Huang, Hongpeng Qin, and Zhuqing Jiang
Appl. Opt. 60(15) 4418-4425 (2021)

Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging based on lateral shearing interferometry

Jianglei Di, Ying Li, Min Xie, Jiwei Zhang, Chaojie Ma, Teli Xi, Enpu Li, and Jianlin Zhao
Appl. Opt. 55(26) 7287-7293 (2016)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved