Abstract

In this work, the design, construction, and testing of the most cost-effective digital lensless holographic microscope to date are presented. The architecture of digital lensless holographic microscopy (DLHM) is built by means of a 3D-printed setup and utilizing off-the-shelf materials to produce a DLHM microscope costing ${\rm US}\$52.82$. For the processing of the recorded in-line holograms, an open-source software specifically developed to process this type of recordings is utilized. The presented DLHM setup has all the degrees of freedom needed to achieve different fields of view, levels of spatial resolution, and 2D scanning of the sample. The feasibility of the presented platform is tested by imaging non-bio and bio samples; the resolution test targets, a section of the head of a Drosophila melanogaster fly, red blood cells, and cheek cells are imaged on the built microscope.

© 2020 Optical Society of America

Cone-shaped optical fiber tip for cost-effective digital lensless holographic microscopy

Brayan Patiño-Jurado, Juan F. Botero-Cadavid, and Jorge Garcia-Sucerquia
Appl. Opt. 59(10) 2969-2975 (2020)

Preprocessing in digital lensless holographic microscopy for intensity reconstructions with enhanced contrast

Heberley Tobon, Carlos Trujillo, and Jorge Garcia-Sucerquia
Appl. Opt. 60(4) A215-A221 (2021)

Digital lensless holographic microscopy: numerical simulation and reconstruction with ImageJ

Carlos Trujillo, Pablo Piedrahita-Quintero, and Jorge Garcia-Sucerquia
Appl. Opt. 59(19) 5788-5795 (2020)

Adapting a Blu-ray optical pickup unit as a point source for digital lensless holographic microscopy

Heberley Tobón-Maya, Alejandra Gómez-Ramírez, Carlos Buitrago-Duque, and Jorge Garcia-Sucerquia
Appl. Opt. 62(10) D39-D47 (2023)

Color digital lensless holographic microscopy: laser versus LED illumination

Jorge Garcia-Sucerquia
Appl. Opt. 55(24) 6649-6655 (2016)