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Fast and pure phase-shifting off-axis holographic microscopy with a digital micromirror device

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Abstract

We present a phase-shifting digital holographic microscopy technique, where a digital micromirror device enables to perform a precise phase-only shift of the reference wave. By coupling the beam into a monomode fiber, we obtain a laser mode with a constant phase shift, equally acting on all pixels of the hologram. This method has the advantage of being relatively simple and compatible with high frame rate cameras, which makes it of great interest for the observation of fast phenomena. We demonstrate the validity of the technique in an off-axis configuration by imaging living paramecia caudata.

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Supplementary Material (4)

NameDescription
Visualization 1       Amplitude image showing the locomotion of paramecia caudata in water and some biological residues. Reconstructed from a series of DMD 4-phase-shifted holograms.
Visualization 2       Phase image showing the locomotion of paramecia caudata in water and some biological residues. Reconstructed from a series of DMD 4-phase-shifted holograms.
Visualization 3       Amplitude image showing the locomotion of ciliates in water. Reconstructed from a series of DMD 4-phase-shifted holograms. The off-axis angle is important to avoid overlapping between the +1 order and the zero order residue.
Visualization 4       Amplitude image showing the locomotion of ciliates in water. Reconstructed from a series of DMD 4-phase-shifted holograms. The off-axis angle is small and the +1 order overlap with the zero order residues.

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Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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Figures (10)

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Equations (7)

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