Abstract

The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.

© 2022 Optica Publishing Group

Effects of refractive index on near-infrared tomography of the breast

Hamid Dehghani, Ben A. Brooksby, Brian W. Pogue, and Keith D. Paulsen
Appl. Opt. 44(10) 1870-1878 (2005)

Quantitative refractive index tomography of millimeter-scale objects using single-pixel wavefront sampling

Uihan Kim, Hailian Quan, Seung Hyeok Seok, Yongjin Sung, and Chulmin Joo
Optica 9(9) 1073-1083 (2022)

Three-dimensional bi-functional refractive index and fluorescence microscopy (BRIEF)

Yi Xue, David Ren, and Laura Waller
Biomed. Opt. Express 13(11) 5900-5908 (2022)

Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging

Kyoohyun Kim, Wei Sun Park, Sangchan Na, Sangbum Kim, Taehong Kim, Won Do Heo, and YongKeun Park
Biomed. Opt. Express 8(12) 5688-5697 (2017)

Internal displacement measurements based on color fluorescent speckle pattern by multispectral stereo-DIC and refractive index correction

Zhenning Chen, Xinqiao Tang, Yinuo Xu, Xinxing Shao, Wei Sun, and Xiaoyuan He
Appl. Opt. 61(22) 6504-6509 (2022)