Abstract
A three-dimensional point spread function experimental estimation method based on the system’s focal plane array spatial local impulse response of a mid-wave infrared microscope is presented. The method uses several out-of-focus two-dimensional point spread function planes to achieve a single three-dimensional point spread function of the whole microscope’s optical spreading, expanding the limits of infrared optical technology by one dimension. This technique includes stages of image acquisition, nonuniformity correction, filtering, and multi-planar reconstruction steps, and its effectiveness is demonstrated on biological sample image restoration by means of a multi-planar refocusing application.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Anselmo Jara, Sergio Torres, and Guillermo Machuca
Appl. Opt. 59(17) 5142-5150 (2020)
Jan Sievers, Ricardo Elsner, Sebastian Bohn, Melanie Schünemann, Heinrich Stolz, Rudolf F. Guthoff, Oliver Stachs, and Karsten Sperlich
Biomed. Opt. Express 13(2) 1087-1101 (2022)
Chong Fan, Guanda Li, and Chao Tao
Appl. Opt. 54(13) 4097-4103 (2015)