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Confocal fluorescence microscopy with high-NA diffractive lens arrays

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Abstract

Traditionally, there is a trade-off between the numerical aperture and field of view for a microscope objective. Diffractive lens arrays (DLAs) with overlapping apertures are used to overcome such a problem. A spot array with an NA up to 0.83 and a pitch of 75 µm is produced by the proposed DLA at a wavelength of 488 nm. By measurement of the fluorescence beads, the DLA-based confocal setup shows the capability of high-resolution measurement over an area of $3\;{\rm{mm}} \times 3\;{\rm{mm}}$ with a $2.5 \times 0.07\;{\rm{NA}}$ objective. Further, the proposed fluorescence microscope is insensitive to optical aberrations, which has been demonstrated by imaging with a simple doublet lens.

© 2021 Optical Society of America

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Data Availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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