Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Issues in Progress Current Issue All Issues Early Posting Feature Issues

Specific refraction-index increments of oxygenated hemoglobin from thalassemia-minor patients are not significantly different than those from healthy individuals

Shin-Shyang Huang, Yang-Hsien Lin, Shang-Ju Wu, and Kung-Bin Sung

Not Accessible

Your library or personal account may give you access

Abstract

The mass and concentration of hemoglobin per erythrocyte are important hematological parameters. Measuring these parameters from intact erythrocytes requires the value of specific refraction-index increment (RII) of oxygenated hemoglobin, which diverges in the literature. Refractive indices of hemoglobin solutions are measured directly by digital holographic microscopy on a microfluidic channel filled with hemoglobin solutions prepared by hemolysis of fresh human erythrocytes and refractive-index standards sequentially. Hemoglobin extracted from thalassemic patients shows 3–4% higher RII than that from healthy volunteers, but the difference is not significant in comparison to inter-subject variations within each group. The quantified RIIs are applied to quantify mean corpuscular hemoglobin mass of blood from 37 human subjects, and results are in accord with standard clinical test results.

© 2022 Optica Publishing Group

Full Article  |  PDF Article
More Like This
Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration

Moritz Friebel and Martina Meinke
Appl. Opt. 45(12) 2838-2842 (2006)

Determining the refractive index of human hemoglobin solutions by Kramers–Kronig relations with an improved absorption model

Jonas Gienger, Hermann Groß, Jörg Neukammer, and Markus Bär
Appl. Opt. 55(31) 8951-8961 (2016)

Calibration-free method to determine the size and hemoglobin concentration of individual red blood cells from light scattering

Konstantin A. Sem’yanov, Pit A. Tarasov, Juhani T. Soini, Alexander K. Petrov, and Valeri P. Maltsev
Appl. Opt. 39(31) 5884-5889 (2000)

View More...

Supplementary Material (1)

NameDescription
Supplement 1       Measured refractive index of PDMS and thickness of microfluidic channels

Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use