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Implementation of miniaturized modular-array fluorescence microscopy for long-term live-cell imaging

Abstract

Fluorescence microscopy imaging of live cells has provided consistent monitoring of dynamic cellular activities and interactions. However, because current live-cell imaging systems are limited in their adaptability, portable cell imaging systems have been adapted by a variety of strategies, including miniaturized fluorescence microscopy. Here, we provide a protocol for the construction and operational process of miniaturized modular-array fluorescence microscopy (MAM). The MAM system is built in a portable size (${{15}}\;{\rm{cm}} \times {{15}}\;{\rm{cm}} \times {{3}}\;{\rm{cm}}$) and provides in situ cell imaging inside an incubator with a subcellular lateral resolution (${\sim}{{3}}\;\unicode{x00B5}{\rm m}$). We demonstrated the improved stability of the MAM system with fluorescent targets and live HeLa cells, enabling long-term imaging for 12 h without the need for external support or post-processing. We believe the protocol could guide scientists to construct a compact portable fluorescence imaging system and perform time-lapse in situ single-cell imaging and analysis.

© 2023 Optica Publishing Group

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Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable requests.

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