Abstract

Ultraviolet (UV) hyperspectral imaging technology is commonly used in the field of atmospheric remote sensing. In recent years, some in-laboratory research has been carried out for substance detection and identification. In this paper, UV hyperspectral imaging technology is introduced into microscopy to better utilize the obvious absorption characteristics of components, such as proteins and nucleic acids in biological tissues in the ultraviolet band. A deep UV microscopic hyperspectral imager based on the Offner structure with ${\rm F}\#$ 2.5, low spectral keystone and smile is designed and developed. A 0.68 numerical aperture microscope objective is designed. The spectral range of the system is from 200 nm to 430 nm; the spectral resolution is better than 0.5 nm; and the spatial resolution is better than 1.3 µm. The K562 cells can be distinguished by transmission spectrum of nucleus. The UV microscopic hyperspectral image of the unstained mouse liver slices showed similar results to the microscopic image after hematoxylin and eosin staining, which could help to simplify the pathological examination process. Both results show a great performance in spatial and spectral detecting capabilities of our instrument, which has the potential for biomedical research and diagnosis.

© 2023 Optica Publishing Group

Wideband, high spectral resolution UV convex grating imaging spectrometer based on an Offner structure

Qingsheng Xue, Jingyao Yang, Liyu Huang, Haoxuan Bai, and Jitao Lu
Appl. Opt. 61(12) 3371-3380 (2022)

Spatially encoded hyperspectral compressive microscope for ultrabroadband VIS/NIR hyperspectral imaging

Lukáš Klein, Jan Touš, and Karel Žídek
Appl. Opt. 62(15) 4030-4039 (2023)

Spatial resolution enhancement for pushbroom-based microscopic hyperspectral imaging

Zhe Jiang, Zekuan Yu, Yue Yu, Zhiyu Huang, Qiushi Ren, and Changhui Li
Appl. Opt. 58(4) 850-862 (2019)

Hyperspectral imaging system based on a single-pixel camera design for detecting differences in tissue properties

Joseph Peller, Faramarz Farahi, and Susan R. Trammell
Appl. Opt. 57(27) 7651-7658 (2018)

AOTF based molecular hyperspectral imaging system and its applications on nerve morphometry

Qingli Li, Dongrong Xu, Xiaofu He, Yiting Wang, Zenggan Chen, Hongying Liu, Qintong Xu, and Fangmin Guo
Appl. Opt. 52(17) 3891-3901 (2013)