Using a random laser to measure the content of protein in skim milk

Suely dos Santos Araújo; Suely dos Santos Araújo; Marcus V. A. Prado; Marcus V. A. Prado; Luis M. G. Abegão; Alessandra A. C. Pagani; José Joatan Rodrigues; José Joatan Rodrigues; Sérgio C. Zílio; Márcio A. R. C. Alencar; Márcio A. R. C. Alencar

doi:10.1364/AO.476652

Applied Optics
Vol. 62,
Issue 8,
pp. C53-C58
(2023)
•https://doi.org/10.1364/AO.476652

Using a random laser to measure the content of protein in skim milk

Suely dos Santos Araújo, Marcus V. A. Prado, Luis M. G. Abegão, Alessandra A. C. Pagani, José Joatan Rodrigues, Sérgio C. Zílio, and Márcio A. R. C. Alencar

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Suely dos Santos Araújo, Marcus V. A. Prado, Luis M. G. Abegão, Alessandra A. C. Pagani, José Joatan Rodrigues, Sérgio C. Zílio, and Márcio A. R. C. Alencar, "Using a random laser to measure the content of protein in skim milk," Appl. Opt. 62, C53-C58 (2023)

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Table of Contents Category
- Optical Devices, Sensors, and Detectors
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About this Article
History
- Original Manuscript: September 27, 2022
- Revised Manuscript: December 11, 2022
- Manuscript Accepted: January 9, 2023
- Published: February 2, 2023
Virtual Issues
Applied Optics Latin America Optics and Photonics 2022 (2022)

Abstract

The luminescence of skim milk samples with distinct protein content doped with rhodamine B was investigated. The samples were excited by a nanosecond laser tuned at 532 nm, and the emission was characterized as a random laser. Its features were analyzed as a function of the protein aggregate content. The results showed a linear correlation between the random laser peak intensity and the protein content. This paper proposes a rapid detection photonic method to evaluate the protein content in skim milk based on the intensity of the random laser emission.

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	Content in % mass	Method
Protein	3.167	Kjeldahl
Fat	0.00	Gerber
Fat	0.0032	Goldfish

Sample	Milk Volume %	Water Volume %	Protein Content in % Mass
Undiluted	100	0	3.167
90% diluted	90	10	2.850
80% diluted	80	20	2.534
70% diluted	70	30	2.217
60% diluted	60	40	1.900
50% diluted	50	50	1.584

Sample	Linewidth (nm)		Peak Wavelength (nm)		Threshold (mW)
Undiluted	46.9(2)^a	12.4(2)^b	599.0(9)^a	591.7(1)^b	21(2)^c	26(3)^d
90 % diluted	47.8(2)^a	12.4(2)^b	600.0(4)^a	591.9(1)^b	21(2)^c	26(3)^d
80 % diluted	47.2(2)^a	12.5(2)^b	603(1)^a	591.9(1)^b	26(3)^c	27(3)^d
70 % diluted	46.4(2)^a	13.6(2)^b	601.9(2)^a	591.7(1)^b	25(3)^c	28(3)^d
60 % diluted	46.2(2)^a	15.2(2)^b	600.7(2)^a	591.9(1)^b	30(3)^c	30(3)^d
50 % diluted	44.4(2)^a	16.0(2)^b	601.3(2)^a	591.9(1)^b	–	32(3)^d

	Content in % mass	Method
Protein	3.167	Kjeldahl
Fat	0.00	Gerber
Fat	0.0032	Goldfish

Sample	Milk Volume %	Water Volume %	Protein Content in % Mass
Undiluted	100	0	3.167
90% diluted	90	10	2.850
80% diluted	80	20	2.534
70% diluted	70	30	2.217
60% diluted	60	40	1.900
50% diluted	50	50	1.584

Marcus V. A. Prado	https://orcid.org/0000-0003-4573-0712
Luis M. G. Abegão	https://orcid.org/0000-0001-5329-6413
Márcio A. R. C. Alencar	https://orcid.org/0000-0003-1436-9901

Abstract

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Tables (3)

Equations (1)

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