Light sheet fluorescence microscopy is a technique used to image an extremely thin section of a biological sample, offering axial resolution ranging from a few hundred nanometers to a few micrometers. Employing a thin sheet of illumination perpendicular to the imaging objective lens, it minimizes photodamage and enables live cellular to full organ imaging. This manuscript presents a novel advancement in lightsheet microscopy by incorporating a reflective prism in the detection path, contrasting earlier methods that have used reflective elements in the illumination path. The authors claim a significant reduction in the spherical aberration associated with reflective illumination using high numerical aperture microscope objectives. With high NA objectives employed in both the illumination and detection light paths, this technique achieves a 3D isotropic resolution of approximately 300 nm, while employing a Bessel beam to generate the thin light sheet during illumination.

---

---

 Add Comment

You must log in to add comments.