Abstract

A method is presented for the analysis of mycotoxins using acetonitrile (90) water (10) extraction, petroleum ether defatting and ferric gel decolorization, chloroform partitioning, and a 1000-fold concentration. Gas chromatography and thin layer chromatography are used to separate and analyze the three most common thichothecene mycotoxins. The analysis has been applied to animal feeds, human foods, and animal tissues. Confirmation of the presence of the mycotoxins is made by IR, NMR, and/or mass spectrometric analysis and/or the chicken embryo test. The sensitivity of the method is 1 ppm for the three trichothecenes, the level of biological effectiveness. The gas chromatographic analysis technique is sensitive to 10 ng of each mycotoxin; the thin layer analysis is sensitive to 100 ng of each compound. Mass spectra may be obtained from 100 ng of each toxin taken from a thin layer chromatogram and the LD₅₀ for chicken embryos is 100 ng/egg for T-2 toxin and diacetoxyscirpenol. Vomitoxin (deoxynivalenol) has not been subjected to the chicken embryo test due to the lack of authentic pure vomitoxin.

Humanoid-shaped WaveFlex biosensor for the detection of food contamination

Ragini Singh, Wen Zhang, Xuecheng Liu, Bingyuan Zhang, and Santosh Kumar
Biomed. Opt. Express 14(9) 4660-4676 (2023)

Determination of the birefringence of an optical fiber by measuring n⁽²⁾(T)

M. P. Cagigal, F. Gonzalez, P. de Castro, and J. Basterrechea
Appl. Opt. 26(1) 32-34 (1987)

Determination of the radii and the concentration ratio in binary mixtures of spherical macromolecules from the measurement of n⁽²⁾(T)

M. P. Cagigal, M. A. Rebolledo, and F. Moreno
Appl. Opt. 23(13) 2091-2096 (1984)

On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor

Jiaming Cao, Xinghua Yang, Pingping Teng, Zhihai Liu, Jun Yang, Jianzhong Zhang, Yu Zhang, Meng Luo, Danheng Gao, Depeng Kong, Siyuan Xia, Enming Zhao, and Libo Yuan
Appl. Opt. 58(21) 5774-5779 (2019)

Synthesis, chemical characterization and nonlinear optical properties of thallium(III) phthalocyanine halide complexes

Jeffrey P. Fitzgerald, Peter D. Huffman, Ian A. Brenner, Jeremiah J. Wathen, Guy Beadie, Richard G. S. Pong, James S. Shirk, and Steven R. Flom
Opt. Mater. Express 5(7) 1560-1578 (2015)