Abstract

Several computer enhancement techniques are compared for their ability to resolve overlapping infrared carbonyl bands of chlorophyll obtained in the solid state. These methods are: the band fit optimization technique, the second-derivative technique, the subtraction of a super-smooth spectrum technique, and the Fourier self-deconvolution technique. The advantages and disadvantages of each technique are compared. In order to extract the maximum information on the spectra, two techniques should be used. The best combination we found was to first use the Fourier self-deconvolution technique, which gives some information on the number and the position of the bands, and—with this information—to then use the band fit optimization technique, which gives the exact intensity, bandwidth, and band shape, as well as the integrated intensity of all the bands.

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