Abstract

The full information of the luminescence intensity as a function of excitation and emission wavenumber is contained in the excitation-emission matrix (EEM). An equal intensity contour plot of this EEM allows a convenient two-dimensional presentation of the experimental findings. In FLN spectra the excitation step selects one or more subsets of molecules which are excited into the <i>S</i>₁ state. Such spectra depend intrinsically on the excitation wavenumber, and their interpretation is greatly facilitated by study of their contour plots. This technique is applied to oxazine-4-perchlorate in PVB, which shows a very broad inhomogeneous width in comparison to its vibrational spacings and which is therefore difficult to interpret with conventional one-dimensional methods.

Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

Sandra Pampín-Suárez, José Luis Arce-Diego, Olga Tapia, Flor María Pérez-Campo, José Carlos Rodríguez-Rey, and Félix Fanjul-Vélez
Biomed. Opt. Express 13(1) 197-208 (2022)

Fourier fluorescence spectrometer for excitation emission matrix measurement

Leilei Peng, Joseph A. Gardecki, Brett E. Bouma, and Guillermo J. Tearney
Opt. Express 16(14) 10493-10500 (2008)

Quantitative FRET measurement by high-speed fluorescence excitation and emission spectrometer

Jing Yuan, Leilei Peng, Brett E. Bouma, and Guillermo J. Tearney
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Quantitative multi-color FRET measurements by Fourier lifetime excitation-emission matrix spectroscopy

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Opt. Express 20(24) 26806-26827 (2012)

Structure and van der Waals vibrational frequencies of the glyoxal · Ar₂ complex studied by fluorescence excitation and stimulated-emission spectroscopy

Donald Frye, Luc Lapierre, and Hai-Lung Dai
J. Opt. Soc. Am. B 7(9) 1905-1914 (1990)