Abstract

A rapid and sensitive method based on measurement of carbon dioxide released from a culture medium as a result of bacterial metabolism of glucose is reported. The rate of release of carbon dioxide was measured by recording the absorbance at 2353 cm⁻¹ (4.25 μm) against time using a standard infrared spectrophotometer. Detection of growth from only five organisms of <i>Salmonella typhosa</i> was significant after just fifteen minutes. In about three hours a clear pattern of growth was obtained by this method. The results are reproducible with a standard deviation around ±0.5%.

Measurement of Cell Thickness in Infrared Spectrophotometry

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Selective detection of bacterial layers with terahertz plasmonic antennas

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A Photographic Method of Spectrophotometry in the Red and Infrared*

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Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

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