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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 49,
  • Issue 2,
  • pp. 224-228
  • (1995)

Time-Dependent Fluorescence Depolarization Analysis in Three-Dimensional Microspectroscopy

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Three-dimensional space- and time-resolved fluorescence depolarization spectroscopy has been developed for elucidating picosecond rotational relaxation processes occurring in micrometer-sized volumes. Anisotropy decays are obtained by a new analytical method which is adapted to the large-solid-angle characteristic of the microscope optics. System parameters introduced in this method were determined by measuring a reference sample. The fluorescence dynamics of <i>p</i>-terphenyl in a thin liquid layer was analyzed as a demonstration experiment.

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