Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 51,
  • Issue 7,
  • pp. 1002-1007
  • (1997)

DNA-Dye Fluorescence Enhancement Based on Shifting the Dimer-Monomer Equilibrium of Fluorescent Dye

Not Accessible

Your library or personal account may give you access

Abstract

In this paper, the investigation of DNA-dye fluorescence enhancement based on shifting the dimer-monomer equilibrium of a fluorescent dye, acridine orange (AO), is reported. Formation of a virtually nonfluorescent dimeric dye, acridine orange homodimer (AOAO), induced by the pre-micellar aggregation of an anionic surfactant, sodium dodecyl sulfate (SDS), was observed. The possibility of using the in situ formed AOAO as a fluorescent probe for nucleic acids and polynucleotides was studied. The results showed that a nearly 1000-fold fluorescence enhancement was observed upon addition of calf thymus DNA (CT DNA). The fluorescence enhancement effect of DNA was thought to be based on the DNA modulated shift of the dimer-monomer equilibrium of AO in the anionic surfactant solution. Intercalation of the monomer in DNA caused the dissociation of AOAO and led to a very high fluorescence enhancement. It seemed that the dimeric dye molecules acted as a source of monomer molecules ready for interacting with nucleic acids and, at the same time, decreased the inherent fluorescence of monomer molecules, which proved to be unfavorable to the detection of fluorescence enhancement. A linear dependence of fluorescence intensity on CT DNA concentration over a range from 7.8 ng/mL to 10.0 mu g/mL, in the presence of AO at a concentration of 1.65 X 10 -6mol/L and of SDS at a concentration of 8.0 X 10-4mol/L , allowed sensitive quantitation of CT DNA in a conventional fluorometer. Calibration graphs for yeast RNA and polynucleotides, such as poly A, poly U, and poly I, were also obtained.

PDF Article
More Like This
Fluorescence enhancement of hemicyanines bound to DNA or DNA-complex and their application to dye laser

Yuki Suzuki and Yutaka Kawabe
Opt. Mater. Express 7(6) 2062-2068 (2017)

Sensitive determination of DNA based on phosphate-dye interaction using photothermal lens technique

Nader Shokoufi, Behnaz Abbasgholi Nejad Asbaghi, and Shafigh Nouri Hajibaba
Appl. Opt. 58(12) 3074-3082 (2019)

Light amplification in DNA-surfactant complex films stained by hemicyanine dye with immersion method

Takemasa Suzuki and Yutaka Kawabe
Opt. Mater. Express 4(7) 1411-1419 (2014)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved