Abstract

Two-dimensional Fourier transform infrared (2D FT-IR) spectroscopy was applied to study the slower states of the membrane protein bacterioRhodopsin (bR) photocycle, with bR adsorbed on a ZnSe attenuated total reflectance (ATR) crystal. The M and the N states of the bR photocycle could be discriminated, and 2D spectra revealed an N state absorption band at 1536 cm^-1 that is normally covered by the broader and bigger 1527 cm^-1 M state absorption band. 2D FT-IR absorbance changes are presented in a more convenient way for analysis than in traditional 2D FT-IR spectroscopy by using 2D-phase spectra and phase/amplitude calculations.

Phenomenological characterization of bacteriorhodopsin–D85N photocycle

Doğan A. Timuçin and John D. Downie
J. Opt. Soc. Am. B 17(2) 202-212 (2000)

Bacteriorhodopsin films as spatial light modulators for nonlinear-optical filtering

R. Thoma, N. Hampp, C. Bräuchle, and D. Oesterhelt
Opt. Lett. 16(9) 651-653 (1991)

Recent applications of FT-IR spectroscopy to polymer systems

J. L. Koenig and M. K. Antoon
Appl. Opt. 17(9) 1374-1385 (1978)

Monitoring intermediate states of bacteriorhodopsin monolayers using near-field optical microscopy

Narasimhan Arun, Sabyasachi Mukhopadhyay, and K. S. Narayan
Appl. Opt. 49(7) 1131-1138 (2010)

All-optical switching characteristics in bacteriorhodopsin and its applications in integrated optics

Yuhua Huang, Shin-Tson Wu, and Youyuan Zhao
Opt. Express 12(5) 895-906 (2004)