Abstract
The combination of FT-IR flow titrations and second-order calibration methods is assessed for the first time as a potential method for quantifying mixtures of organic acids. FT-IR spectral information is richer and more specific than that provided by other spectrometric techniques. Also, the flow titration of the sample allows the use of second-order data analysis methods, such as multivariate curve resolution, which can quantify multianalyte systems using pure analyte standards in the presence of unknown interferents. Problems linked to FT-IR flow titrations, such as the impossibility of using buffer substances and the presence of major baseline contributions in the measurements due to changes in the water structureless absorption, have been overcome through the design of an adapted experimental setup and the use of second derivative spectra. Malic and/or tartaric acid are the model substances chosen to be quantified in the presence or absence of interferents. Both substances are diprotic acids with similar p<i>K</i><sub>a</sub> values and, therefore, similar pH-dependent evolution. Samples with one or both acids with their related standards in the presence or absence of an inert interferent (sugar) are successfully resolved and quantified for a wide range of [analyte]:[interferent] ratios with no need for pH control in the process. These results suggest this methodology as an optimal and robust alternative for routine analysis.
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