Abstract
We report a methodology that allows identification and quantitative determination of individual acidic and neutral diterpenes in natural mixtures, using the computer-aided analysis of their <sup>13</sup>C-NMR spectra. The analytical procedure was validated on artificial mixtures and then applied to authentic oleoresins of <i>Pinus nigra</i>. Up to eleven diterpenes, which represented 63% of the total composition of the oleoresin, were identified and quantified without previous separation or derivatization.
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