Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 57,
  • Issue 11,
  • pp. 1407-1418
  • (2003)

Stopped Flow Apparatus for Time-Resolved Fourier Transform Infrared Difference Spectroscopy of Biological Macromolecules in 1H2O

Not Accessible

Your library or personal account may give you access

Abstract

Stopped flow spectroscopy is an established technique for acquiring kinetic data on dynamic processes in chemical and biochemical reactions, and Fourier transform infrared (FT-IR) techniques can provide particularly rich structural information on biological macromolecules. However, it is a considerable challenge to design an FT-IR stopped flow system with an optical path length low enough for work with aqueous (<sup>1</sup>H<sub>2</sub>O) solutions. The system presented here is designed for minimal sample volumes (~5 μL) and allows simultaneous FT-IR rapid-scan and VIS measurements. The system employs a micro-structured diffusional mixer to achieve effective mixing on the millisecond time scale under moderate flow and pressure conditions, allowing measurements in a cell path length of less than 10 μm. This makes it possible to record spectra in <sup>1</sup>H<sub>2</sub>O solutions over a wide spectral range. The system layout is also designed for a combination of kinetic and static measurements, in particular to obtain detailed information on the faster spectral changes occurring during the system dead time. A detailed characterization of the FTIR stopped flow system is presented, including a demonstration of the alkaline conformational transition of cytochrome <i>c</i> as an example.

PDF Article
More Like This
Time-resolved Fourier transform intracavity spectroscopy with a Cr2+:ZnSe laser

Nathalie Picqué, Fatou Gueye, Guy Guelachvili, Evgeni Sorokin, and Irina T. Sorokina
Opt. Lett. 30(24) 3410-3412 (2005)

Time-resolved continuous-filtering Vernier spectroscopy of H2O and OH radical in a flame

Chuang Lu, Francisco Senna Vieira, Florian M. Schmidt, and Aleksandra Foltynowicz
Opt. Express 27(21) 29521-29533 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved