Abstract

The solid-matrix fluorescence (SMF) quenching of benzo[e]pyrene and (±)-anti-dibenzo[a, l]pyrene-11,12-diol-13,14-epoxide ((±)-antiDB[a, l]PDE)-DNA adducts with thallium nitrate (TlNO₃) and sodium iodide (NaI) was examined and several SMF quenching models were developed. The SMF quenching data for B[e]P with either TlNO₃ or NaI fit a two-independent-binding-site model. However, the SMF quenching of (±)-anti-DB[a, l]PDE-DNA adducts with TlNO₃ fits a sphere of action model, but quenching with NaI was modeled with the two-independent-binding-site model. The data were compared with earlier SMF quenching data for 7,8,9,10-tetrahydroxytetrahydro-benzo[a]pyrene (tetrol I-1) and (±)-anti-benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide ((±)-anti-BPDE)DNA adducts. The interpretation of the SMF quenching data for the (±)-anti-DB[a, l]PDE-DNA adducts was distinctively different than the interpretation of the SMF quenching data for the (±)-antiBPDE-DNA adducts. This initial study shows that SMF quenching has the potential to characterize polycyclic aromatic hydrocarbonDNA adducts with different numbers of aromatic rings. In addition, the data indicated that external and intercalated DNA adducts interacted with heavy-atom salts in dissimilar fashions. The new SMF methodology developed is useful for the characterization of both polycyclic aromatic hydrocarbon-DNA adducts and metabolites from polycyclic aromatic hydrocarbons.

Flame temperature measurements using the anomalous fluorescence of pyrene

Diane L. Peterson, Fred E. Lytle, and Normand M. Laurendeau
Appl. Opt. 27(13) 2768-2775 (1988)

Detection of oxygen by fluorescence quenching

M. E. Cox and B. Dunn
Appl. Opt. 24(14) 2114-2120 (1985)

Determination of flame temperature using the anomalous fluorescence of pyrene

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Opt. Lett. 11(6) 345-347 (1986)

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