Abstract

The two-point resolution of a novel two-color far-field super-resolution fluorescence microscopy was evaluated by measuring fluorescent beads 100 nm in diameter. This microscopy is based on a combination of two-color fluorescence dip spectroscopy and a phase-modulation technique for a laser beam. By simply introducing two-color laser light, the size of the fluorescent image of a bead was shrunk down to a diameter of 250 nm from the diffraction-limited image with a diameter of 360 nm. For two closely adjacent fluorescent beads with a separation distance of 350 nm, the two-color microscope clearly gave separated fluorescence images, while the conventional one-color fluorescence microscope could not resolve them. It has been proved that our technique breaks Rayleigh's diffraction limit.

Two-point-separation in super-resolution fluorescence microscope based on up-conversion fluorescence depletion technique

Takeshi Watanabe, Yoshinori Iketaki, Takashige Omatsu, Kimihisa Yamamoto, Makoto Sakai, and Masaaki Fujii
Opt. Express 11(24) 3271-3276 (2003)

Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy

Keiichi Inoue, Nándor Bokor, Satoshi Kogure, Masaaki Fujii, and Makoto Sakai
Opt. Express 17(14) 12013-12018 (2009)

Creation of a three-dimensional spherical fluorescence spot for super-resolution microscopy using a two-color annular hybrid wave plate

Yoshinori Iketaki, Hiroshi Kumagai, Kornel Jahn, and Nandor Bokor
Opt. Lett. 40(6) 1057-1060 (2015)

Subdiffraction resolution in far-field fluorescence microscopy

Thomas A. Klar and Stefan W. Hell
Opt. Lett. 24(14) 954-956 (1999)

Note on the classification of super-resolution in far-field microscopy and information theory

Oliver Passon and Johannes Grebe-Ellis
J. Opt. Soc. Am. A 33(7) B31-B35 (2016)