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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 66,
  • Issue 3,
  • pp. 289-293
  • (2012)

Phosphorylation Detection and Characterization in Ribonucleotides Using Raman and Raman Optical Activity (ROA) Spectroscopies

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Abstract

<b>Raman and Raman optical activity (ROA) spectra are presented for adenosine and seven of its derivative ribonucleotides. Both of these spectroscopic techniques are shown to be sensitive to the site and degree of phosphorylation, with a considerable number of marker bands being identified for these ribonucleotides. ROA spectra are shown to provide the most sensitive diagnostic tool for phosphorylation characterization and quantification.</b>

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