Abstract

The ultraviolet absorption spectra of organic compounds which have an acidic or basic functional group as an integral part of the chromophoric system will generally be pH-dependent; that is, the form and intensity of the absorption curve will change as the hydrogen ion concentration changes. This property may be utilized in the determination of dissociation constants of an acid or base by spectrophotometry.

Homochromatic Spectrophotometry in the Extreme Ultraviolet

George R. Harrison and Philip A. Leighton
J. Opt. Soc. Am. 20(6) 313-330 (1930)

Test Methods in Spectrophotometry: Stray-Light Determination

Richard E. Poulson
Appl. Opt. 3(1) 99-104 (1964)

Ultraviolet Spectrophotometry: Comments on Apparatus And Experimental Methods*

George E. Davis and Charles Sheard
J. Opt. Soc. Am. 21(1) 47-58 (1931)

The Ultraviolet Absorption Spectra of Protein Solutions1

R. Norman Jones and Hugh J. Creech
J. Opt. Soc. Am. 33(4) 209-218 (1943)

Organic Media as Spectral Filters in the Ultraviolet1

Thos. M. Dahm
J. Opt. Soc. Am. 15(5) 266-284 (1927)