Abstract

In the following work, the vibrational spectroscopic characteristics of artepillin C are reported by means of Fourier transform infrared (FT-IR) and Raman spectroscopies, surface-enhanced Raman scattering (SERS), and coherent anti-Stokes Raman scattering (CARS) microscopy. Artepillin C is an interesting compound due to its pharmacological properties, including antitumor activity. It is found as the major component of Brazilian green propolis, a resinous mixture produced by bees to protect their hives against intruders. Vibrational spectroscopic techniques have shown a strong peak at 1599 cm⁻¹, assigned to C=C stretching vibrations from the aromatic ring of artepillin C. From these data, direct visualization of artepillin C could be assessed by means of CARS microscopy, showing differences in the film hydration obtained for its neutral and deprotonated states. Raman-based methods show potential to visualize the uptake and action of artepillin C in biological systems, triggering its interaction with biological systems that are needed to understand its mechanism of action.

© 2020 The Author(s)

High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy

Feruz Ganikhanov, Conor L. Evans, Brian G. Saar, and X. Sunney Xie
Opt. Lett. 31(12) 1872-1874 (2006)

Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera

Yusuke Murakami, Minami Yoshimura, W. J. Niels Klement, Atsuki Oda, Ryo Sakamoto, Miho Yakabe, Atsushi Matsumoto, Ryosuke Oketani, Philippe Leproux, Junichi Ikenouchi, Wesley R. Browne, and Hideaki Kano
Opt. Continuum 2(9) 2044-2054 (2023)

Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging

Eric O. Potma, Conor L. Evans, and X. Sunney Xie
Opt. Lett. 31(2) 241-243 (2006)

Vibrational spectroscopy and imaging with non-resonant coherent anti-Stokes Raman scattering: double stimulated Raman scattering scheme

Dae Sik Choi, Chang Ho Kim, Taegon Lee, Sanghee Nah, Hanju Rhee, and Minhaeng Cho
Opt. Express 27(16) 23558-23575 (2019)

Spectral focusing dual-comb coherent anti-Stokes Raman spectroscopic imaging

Kun Chen, Tao Wu, Tao Chen, Haoyun Wei, Honglei Yang, Tian Zhou, and Yan Li
Opt. Lett. 42(18) 3634-3637 (2017)