Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Single-Molecule Fluorescence Techniques for Membrane Protein Dynamics Analysis

Not Accessible

Your library or personal account may give you access

Abstract

Fluorescence-based single-molecule techniques, mainly including fluorescence correlation spectroscopy (FCS) and single-molecule fluorescence resonance energy transfer (smFRET), are able to analyze the conformational dynamics and diversity of biological macromolecules. They have been applied to analysis of the dynamics of membrane proteins, such as membrane receptors and membrane transport proteins, due to their superior ability in resolving spatio-temporal heterogeneity and the demand of trace amounts of analytes. In this review, we first introduced the basic principle involved in FCS and smFRET. Then we summarized the labeling and immobilization strategies of membrane protein molecules, the confocal-based and TIRF-based instrumental configuration, and the data processing methods. The applications to membrane protein dynamics analysis are described in detail with the focus on how to select suitable fluorophores, labeling sites, experimental setup, and analysis methods. In the last part, the remaining challenges to be addressed and further development in this field are also briefly discussed.

© 2021 The Author(s)

PDF Article
More Like This
Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy

Matteo Bernardello, Radoslaw J. Gora, Patrick Van Hage, Gustavo Castro-Olvera, Emilio J. Gualda, Marcel J. M. Schaaf, and Pablo Loza-Alvarez
Biomed. Opt. Express 12(10) 6205-6227 (2021)

Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy

Yuanqing Ma, Aleš Benda, Philip R. Nicovich, and Katharina Gaus
Biomed. Opt. Express 7(4) 1561-1576 (2016)

A Bayesian method for single molecule, fluorescence burst analysis

P. R. Barber, S. M. Ameer-Beg, S. Pathmananthan, M. Rowley, and A. C. C. Coolen
Biomed. Opt. Express 1(4) 1148-1158 (2010)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved