Abstract

The global fight against mosquito-borne viral diseases has in recent years been bolstered by the introduction of the endosymbiotic bacteria Wolbachia to vector populations, which in host mosquitoes suppresses the transmissibility of several viruses. Researchers engaged on this front of the battle often need to know the Wolbachia infection status of individual mosquitoes, as the intervention progresses and the mosquitoes become established in the target population. Previously, we successfully applied attenuated total reflection Fourier transform infrared spectroscopy to the detection of Wolbachia in adult Aedes aegypti mosquitoes; here we apply the same principles to Aedes eggs, with sensitivity and selectivity > 0.95. Further, we successfully distinguish between infections in eggs of the wMel and wMelPop strains of Wolbachia pipientis, with a classification error of 3%. The disruption of host lipid profile by Wolbachia is found to be a key driver in spectral differences between these sample classes.

© 2021 The Author(s)

Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures

Mateo R. Hernandez, Elyse N. Towns, Terry C. Ng, Brian C. Walsh, Richard Osibanjo, Atul N. Parikh, and Donald P. Land
Appl. Opt. 51(15) 2842-2846 (2012)

Chemical visualization of individual chondrocytes in articular cartilage by attenuated-total-reflection Fourier Transform Infrared Microimaging

Jianhua Yin and Yang Xia
Biomed. Opt. Express 2(4) 937-945 (2011)

Detection of colonic inflammation with Fourier transform infrared spectroscopy using a flexible silver halide fiber

Vinay K. Katukuri, John Hargrove, Sharon J. Miller, Kinan Rahal, John Y. Kao, Rolf Wolters, Ellen M. Zimmermann, and Thomas D. Wang
Biomed. Opt. Express 1(3) 1014-1025 (2010)

Label-free identification of individual bacteria using Fourier transform light scattering

YoungJu Jo, JaeHwang Jung, Min-hyeok Kim, HyunJoo Park, Suk-Jo Kang, and YongKeun Park
Opt. Express 23(12) 15792-15805 (2015)

Dielectric property measurement of human sweat using attenuated total reflection terahertz time domain spectroscopy

Kazuma Hashimoto, Paul Ben Ishai, Erik Bründermann, and Saroj R. Tripathi
Biomed. Opt. Express 13(9) 4572-4582 (2022)