Abstract

The reconstruction of the three-dimensional (3D) morphology of polymeric microsphere layers based on confocal Raman microscopy was studied. Refraction of the Raman laser beam at the curved surface of the spheres broadens the focus volume inside the sphere. Compared to planar layers, the focus gets trapped inside the spheres such that the measured depth profiles are shifted and broadened. Additionally, the Raman signal of the underlying substrate is already observed for nominal focus positions above the microsphere layer. The results are successfully modeled with ray-optical simulations that allow for a clear understanding of the relevant mechanisms that lead to the generation of the Raman signals in the complex three-dimensional structures.

© 2022 The Author(s)

Three-dimensional materials analysis by confocal Raman microspectroscopy

Lothar Kador, Tobias Schittkowski, Markus Bauer, and Yuwei Fan
Appl. Opt. 40(28) 4965-4970 (2001)

Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

Lisong Yang, Cian M. Taylor, Yury Rakovich, and Eithne M. McCabe
Appl. Opt. 42(28) 5693-5700 (2003)

Three-dimensional confocal microscopy of colloids

A. D. Dinsmore, Eric R. Weeks, Vikram Prasad, Andrew C. Levitt, and D. A. Weitz
Appl. Opt. 40(24) 4152-4159 (2001)

Three-dimensional reconstruction of topological deformation in chiral nematic microspheres using fluorescence confocal polarizing microscopy

Jin-Kun Guo and Jang-Kun Song
Opt. Express 24(7) 7381-7386 (2016)

Three-dimensional imaging with reflection synthetic confocal microscopy

Md Rasedujjaman, Kévin Affannoukoué, Nicolas Garcia-Seyda, Philippe Robert, Hugues Giovannini, Patrick C. Chaumet, Olivier Theodoly, Marie-Pierre Valignat, Kamal Belkebir, Anne Sentenac, and Guillaume Maire
Opt. Lett. 45(13) 3721-3724 (2020)