Abstract
Photobiomodulation (PBM) using organic light emitting diodes (OLEDs)
surface light sources have recently been claimed to be the next generation
of PBM light sources. However, the differences between light emitting
diodes (LEDs) and OLED mechanisms in vitro and in vivo have not been well
studied. In vivo mouse models were used to investigate the effects of OLED
irradiation on cellular function and cutaneous wound healing compared to
LED irradiation. Mice in the LED- and OLED-irradiated groups were
subjected to irradiation with 6 J/cm2 LED and OLED (630 nm),
respectively, for 14 days after wounding, and some mice were sacrificed
for the experiments on days 3, 7, 10, and 14. To evaluate wound healing,
we performed hematoxylin-eosin and Masson’s trichrome staining and
quantified collagen density by computerized image analysis. The results
showed that the size of the wound, collagen density, neo-epidermis
thickness, number of new blood vessels, and number of fibroblasts and
neutrophils was significantly influenced by LED and OLED irradiation. The
tissue levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor
(TNF)-α were investigated by immunohistochemical staining. LED and OLED
irradiation resulted in a significant increase in the tissue IL-1β and
IL-6 levels at the early stage of wound healing (P < 0.01), and a
decrease in the tissue TNF-α level at all stages of wound healing (P <
0.05), compared to the no-treatment group. The expression levels of the
genes encoding vascular endothelial growth factor and transforming growth
factor-beta 1 were significantly increased in LED and OLED-irradiated
wound tissue at the early stage of wound healing (P < 0.01) compared to
the no-treatment group. Thus, OLED as well as LED irradiation accelerated
wound healing by modulating the synthesis of anti-inflammatory cytokines
and the expression levels of genes encoding growth factors, promoting
collagen regeneration and reducing scarring. In conclusion, this suggests
the possibility of OLED as a new light source to overcome the limitations
of existing PBMs.
© 2019 Optical Society of Korea
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